- MA5-15884 - Invitrogen Antibodies KRT19 antibody

Submitted references Tetrahydroxylated bile acids improve cholestatic liver and bile duct injury in the Mdr2(-/-) mouse model of sclerosing cholangitis via immunomodulatory effects.

Fuchs CD, Dixon ED, Hendrikx T, Mlitz V, Wahlström A, Ståhlman M, Scharnagl H, Stojakovic T, Binder CJ, Marschall HU, Trauner M
Hepatology communications 2022 Sep;6(9):2368-2378

Ulipristal Acetate Interferes With Actin Remodeling Induced by 17β-Estradiol and Progesterone in Human Endometrial Stromal Cells.

Shortrede JE, Montt-Guevara MM, Pennacchio G, Finiguerra M, Giannini A, Genazzani AD, Simoncini T
Frontiers in endocrinology 2018;9:350

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Western blot analysis of KRT19 using a KRT19 monoclonal antibody (Product # MA5-15884) against a human KRT19 (AA: 115-269) recombinant protein.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of KRT19 using a KRT19 monoclonal antibody (Product # MA5-15884) against a human KRT19 (AA: 115-269) recombinant protein.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Western blot analysis of KRT19 using KRT19 monoclonal antibody (Product # MA5-15884) in T47D (1), MCF-7 (2), HepG2 (3) and SW620 (4) cell lysate.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Knockdown of KRT19 was achieved by transfecting MCF-7 with KRT19 specific siRNAs (Silencer® select Product # s7998). Western blot analysis (Fig. a) was performed using membrane enriched extracts from the KRT19 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with Cytokeratin 19 Monoclonal Antibody (4E8) (Product # MA5-15884, 1:1000 dilution) and Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 0.25µg/ml, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to KRT19.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Western blot was performed using Anti-Cytokeratin 19 Monoclonal Antibody (4E8) (Product # MA5-15884) and a 44 kDa band corresponding to KRT19 was observed across cell lines tested. Membrane enriched extracts (30 µg lysate) of MCF-7 (Lane 1), SK-BR-3 (Lane 2), T-47D (Lane 3), COLO 205 (Lane 4) and U-2 OS (Lane 5) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Immunofluorescence analysis of HepG2 cells using KRT19 monoclonal antibody (Product # MA5-15884) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: actin filaments have been labeled with phalloidin.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of Cytokeratin 19 was performed using 70% confluent log phase MCF-7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Cytokeratin 19 Mouse Monoclonal Antibody (4E8) (Product # MA5-15884) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using KRT19 monoclonal antibody (Product # MA5-15884) followed with DAB staining.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Immunohistochemical analysis of paraffin-embedded human colon cancer tissues using KRT19 monoclonal antibody (Product # MA5-15884) followed with DAB staining.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical analysis of paraffin-embedded human stomach cancer tissues using KRT19 monoclonal antibody (Product # MA5-15884) followed with DAB staining.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Immunohistochemical analysis of paraffin-embedded human bladder cancer tissues using KRT19 monoclonal antibody (Product # MA5-15884) followed with DAB staining.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Figure 1 Isolation and characterization of human endometrial cells. Cells were obtained from endometrial biopsies and cultured in DMEM-F12 supplemented with 10% FBS. (A) Phase contrast microscopy of Endometrial Stromal Cells (ESC), cells appeared flattened and elongated with a fibroblast-like shape. Photo taken 4 day after plating (20X). (B) Phase contrast microscopy of EEC cells appeared cuboidal. Photo was taken 4 days after plating. (C) Representative western-blot blot of Vimentin (VMT), Cytokeratin 19 (KRT-19), Estrogen Receptor alpha (ERalpha), Progesterone Receptor (PR), and GAPDH. ESC were VMT/ERalpha/PR positive. EEC were KRT-19/ERalpha/PR positive. GAPDH was used as loading control.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: 4 FIGURE Tetrahydroxylated bile acid (THBA) feeding improves liver and bile duct injury in the Mdr24 -/- mouse model of sclerosing cholangitis. (A) Representative H&E images (x10 magnification) with improved liver histology in Mdr2 -/- mice fed a 0.5% wt/wt THBA-enriched diet for 4 weeks. (B) Serum biochemistry reflects reduced levels of transaminases (ALT, AST) Mdr2 -/- mice fed 0.5% wt/wt THBA. Total bile acid (BA) levels as well as ALP levels tended to be reduced due to THBA feeding. (C) Representative MAC-2 immunohistochemistry images (x10 magnification) showing reduced numbers of macrophages in the livers of THBA-fed Mdr2 -/- mice. (D) Real-time PCR was used to assess the mRNA expression of F4/80 , as markers of inflammation, which was reduced in THBA-fed Mdr2 -/- mice. (E) Representative sirius red stainings (x10 magnification) show tendentially reduced biliary fibrosis in Mdr2 -/- mice fed 0.5% wt/wt THBA. (F) Real-time PCR was used to assess the mRNA expression of fibrotic marker collagen type I alpha 1 ( Col1a1) , which was reduced in THBA-fed Mdr2 -/- mice. (G) Representative CK19 immunohistochemistry pictures (x10 magnification) show reduced cholangiocyte proliferation in THBA-fed Mdr2 -/- mice. (H) Real-time PCR was used to assess the mRNA expression of cholangiocyte proliferation marker CK19 , which was reduced in THBA-fed Mdr2 -/- mice. mRNA expression values were normalized against 36b4 levels and are shown relative to expression level in WT controls. *Signific

MA5-15884

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