Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- TA319484 - Provider product page
- Provider
- OriGene
- Product name
- Goat polyclonal anti-POLK antibody
- Antibody type
- Polyclonal
- Description
- Goat polyclonal anti-POLK antibody
- Host
- Goat
- Conjugate
- Unconjugated
- Epitope
- POLK
- Antibody clone number
- NULL
- Vial size
- 100 µg
- Concentration
- 1.4 mg/mL
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- WB using Anti-POLK antibody shows detection of a band ~98 kDa corresponding to human POLK (arrowhead).? Approximately 35 ug of a HeLa whole cell lysate was separated by 4-20% SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,000. Reaction occurred overnight at 4? followed by washes and reaction with a 1:20,000 dilution of IRDye?800 conjugated Rb-a-Goat IgG [H&L] for 45 min at room temperature.
- Validation comment
- WB