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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [4]
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- Western blot [1]
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- Product number
- NB300-278 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB300-278, RRID:AB_10002954
- Product name
- Rabbit Polyclonal PLK1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specific for the ~120k PLKK protein phosphorylated at Ser482,486,490.
- Reactivity
- Xenopus
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references The polo box is required for multiple functions of Plx1 in mitosis.
A feedback loop in the polo-like kinase activation pathway.
Purification and molecular cloning of Plx1, a Cdc25-regulatory kinase from Xenopus egg extracts.
Purification and molecular cloning of Plx1, a Cdc25-regulatory kinase from Xenopus egg extracts.
Liu J, Lewellyn AL, Chen LG, Maller JL
The Journal of biological chemistry 2004 May 14;279(20):21367-73
The Journal of biological chemistry 2004 May 14;279(20):21367-73
A feedback loop in the polo-like kinase activation pathway.
Erikson E, Haystead TA, Qian YW, Maller JL
The Journal of biological chemistry 2004 Jul 30;279(31):32219-24
The Journal of biological chemistry 2004 Jul 30;279(31):32219-24
Purification and molecular cloning of Plx1, a Cdc25-regulatory kinase from Xenopus egg extracts.
Kumagai A, Dunphy WG
Science (New York, N.Y.) 1996 Sep 6;273(5280):1377-80
Science (New York, N.Y.) 1996 Sep 6;273(5280):1377-80
Purification and molecular cloning of Plx1, a Cdc25-regulatory kinase from Xenopus egg extracts.
Kumagai A, Dunphy WG
Science (New York, N.Y.) 1996 Sep 6;273(5280):1377-80
Science (New York, N.Y.) 1996 Sep 6;273(5280):1377-80
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Western Blot: PLK1 [p Ser482, p Ser486, p Ser490] Antibody [NB300-278] - Analysis of rat hippocampal membrane lysate showing specific immunolabeling of the ~120 kDa Polo-Like Kinase Kinase phosphorylated at Ser482,486,490 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase.
