Antibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
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Validation data
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- Product number
- MA5-15113 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PLK1 Monoclonal Antibody (E.456.6)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- It is not recommended to aliquot this antibody.
- Reactivity
- Human, Rat
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- E.456.6
- Vial size
- 100 µL
- Concentration
- 38 µg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of Serine/threonine-protein kinase PLK1 was achieved by transfecting HeLa with Serine/threonine-protein kinase PLK1 specific siRNAs (Silencer® select Product # s449). Western blot analysis (Fig. a) was performed using Whole cell extracts from the Serine/threonine-protein kinase PLK1 knockdown cells (lane 3), non-targeting scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with PLK1 Monoclonal Antibody (E.456.6) (Product # MA5-15113, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). The decrease in signal upon siRNA mediated knockdown confirms that antibody is specific to Serine/threonine-protein kinase PLK1.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-PLK1 Monoclonal Antibody (E.456.6) (Product # MA5-15113) and a 62kDa band corresponding to Serine/threonine-protein kinase PLK1 was observed across cell lines tested. Whole cell extracts (30 µg lysate) of SW480 (Lane 1), K-562 (Lane 2), MDA-MB-231 (Lane 3) and Hep G2 (Lane 4) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Reagent Kit (Product # WP20005).