Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- AM33053PU-S - Provider product page
- Provider
- OriGene
- Product name
- Mouse Monoclonal Anti-PLK1 Antibody, Purified
- Antibody type
- Monoclonal
- Description
- Mouse Monoclonal Anti-PLK1 Antibody, Purified
- Reactivity
- Human, Mouse
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- Clone 3F8
- Vial size
- 25ug
- Concentration
- 0.5 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western Blot using PLK1 Antibody (Clone 3F8): Hela cell extract (Lane 1) or NIH3T3 cell extract (Lane 2) was resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal anti-Plk-1 (Clone 3F8) antibody. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescent microscope analysis of nocodazole treated Hela cells (10mM for overnight), using PLK1 Antibody (Clone 3F8)? (green). Thr210-phosphorylated PLK-1 (poly6186) has been labeled with red and nuclei were stain with DAPI (blue).
- Validation comment
- IF