R1543P

antibody from Acris Antibodies GmbH

Targeting: PLK1 PLK

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

R1543P

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#R1543P, RRID:AB_1006510

Product name

anti PLK1 pThr210

Antibody type

Polyclonal

Antigen

Synthetic phospho peptide corresponding aa 205-214 of Human Polo-like kinase 1 (Plk1) protein.

Reactivity

Human, Mouse, Rat, Canine, Chicken/Avian, Porcine, Xenopus, Zebrafish

Host

Rabbit

Vial size

0.1 mg

Concentration

1.0 mg/ml (by UV absorbance at 280 nm)

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Western blot analysis is shown using affinity purified R1543P Plk-1 pThr210 antibody to detect endogenous protein present in a Mouse A20 whole cell lysate (arrowhead). Comparison to a Molecular weight marker indicates a band of ~68 kDa corresponding to Plk-1 protein. It is suggested to use a nuclear extract from synchronized cells to greatly increase the abundance of this protein in preparations. The blot was incubated with a 1/500 dilution of the antibody at room temperature followed by detection using standard techniques. Personal communication Steven Pelech, Kinexus Inc. Vancouver, BC.

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Western blot analysis is shown to detect endogenous and recombinant protein present in HeLa cell lysates transfected with various plk-1 mutation constructs. Blots were reacted with anti-Plk-1 pThr210 (Panel A) and pan reactive anti-Plk-1 (Panel B). Transfected cells were treated with 1 µM nocodazole followed by cell collection, lysate preparation, SDS-PAGE and Western blotting. Using a 1/1000 dilution, anti-Plk-1 pThr210 detects a single band corresponding to endogenous plk-1, but does not detect recombinant forms of the protein presumably because of a lack of phosphorylation in these mutants. Personal communication Hai Jiang, Northwestern Univ.

Immunohistochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Formalin-Fixed, Paraffin-Embedded Human Breast carcinoma tumor stained with PLK1 Antibody (pThr210) Cat.-No R1543P at 1/200.

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

R1543P Plk1 antibody pThr210 staining of Formalin-Fixed, Praffin Embedded Human colon carcinoma tumor tissue at 1/200 dilution to detect Plk1 by immunohistochemistry. Personal Communication, Alan Yen, LifeSpanBiosciences, Seattle, WA.