NBP2-59776

antibody from Novus Biologicals

Targeting: COX4I1 COX4, COX4-1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP2-59776

Provider

Novus Biologicals

Product name

Mouse Monoclonal COX4 Antibody

Antibody type

Monoclonal

Description

Protein A purified. Specificity of human, rat COX4 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins

Reactivity

Human, Mouse, Rat

Host

Mouse

Antigen sequence

VVKSEDFSLP


Isotype

IgG

Vial size

100 ul

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: COX4 Antibody (1327) [NBP2-59776] - Analysis of extracts from U-251 cells, transfected with: control siRNA, target specific siRNA probe #1, target specific siRNA probe #2, using Anti-COX4I1 monoclonal antibody. Downregulation of antibody signal confirms target specificity. Remaining % intensity, relative control lane, is indicated. Anti-GAPDH monoclonal antibody was used as loading control.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: COX4 Antibody (1327) [NBP2-59776] - Analysis in human cell line HeLa, human cell line HEK 293, human cell line A-431, human cell line HepG2, mouse cell line NIH-3T3 and rat cell line NBT-II.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: COX4 Antibody (1327) [NBP2-59776] - Staining of human kidney shows strong cytoplasmic positivity in renal tubules and glomerulus cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: COX4 Antibody (1327) [NBP2-59776] - Staining of human stomach shows strong cytoplasmic immunoreactivity in glandular epithelium cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: COX4 Antibody (1327) [NBP2-59776] - Staining of human prostate shows strong cytoplasmic immunoreactivity in epithelial cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: COX4 Antibody (1327) [NBP2-59776] - Staining of human fallopian tube shows strong cytoplasmic positivity in epithelial cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: COX4 Antibody (1327) [NBP2-59776] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.