Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-21359 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- COX4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, H1299, HeLaS3, HepG2, Molt-4, Raji.
- Concentration
- 0.38 mg/mL
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of COX4 using 30 µg of A) A431 and B) MOLT4 lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a COX4 polyclonal antibody (Product # PA5-21359) at a dilution of 1:2000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of COX4 was performed by separating 30 µg of various whole cell extracts by 15% SDS-PAGE. Proteins were transferred to a membrane and probed with a COX4 Polyclonal Antibody (Product # PA5-21359) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of COX4 in methanol-fixed HeLa cells using a COX4 polyclonal antibody (Product # PA5-21359) (Green) at a 1:500 dilution. Alpha-tubulin filaments were labeled with Product # PA5-29281 (Red) at a 1:2000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using COX4 (Product # PA5-21359) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Product # MA1-25054) (Red) at 1:2,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded CA922 xenograft, using COX4 (Product # PA5-21359) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of COX4 was performed in 293T whole cell extracts using 5 µg of COX4 Polyclonal Antibody (Product # PA5-21359). Samples were transferred to a membrane and probed with COX4 Polyclonal Antibody as a primary antibody and an HRP-conjugated anti-Rabbit IgG was used as a secondary antibody.