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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [3]
- Flow cytometry [1]
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Validation data
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- Product number
- TA500961 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA500961, RRID:AB_11125435
- Product name
- Anti-BHMT mouse monoclonal antibody, clone OTI1E5 (formerly 1E5)
- Antibody type
- Monoclonal
- Description
- Anti-BHMT mouse monoclonal antibody, clone OTI1E5 (formerly 1E5)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- BHMT
- Isotype
- IgG
- Antibody clone number
- OTI1E5
- Vial size
- 100 µl
- Concentration
- 0.7 mg/ml
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BHMT (RC203148, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BHMT.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BHMT (RC203148, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BHMT.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-BHMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500961, Dilution 1:50)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-BHMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500961, Dilution 1:50)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of liver tissue using anti-BHMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500961, Dilution 1:50)
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either pCMV6-ENTRY BHMT(RC203148)(Red) or empty vector control plasmid(Blue) were immunostained with anti-BHMT mouse monoclonal(TA500961), and then analyzed by flow cytometry.
- Validation comment
- FC
