PA5-52014

antibody from Invitrogen Antibodies

Targeting: ADAR ADAR1, G1P1, IFI4

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-52014

Provider

Invitrogen Antibodies

Product name

ADAR Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: SDNQPEGMIS ESLDNLESMM PNKVRKIGEL VRYLNTNPVG GLLEYARSHG FAAEFKLVDQ SGPPHEPKFV YQAKVGGRWF PAVCAHSKKQ GKQEAADAAL RVLIGENEKA ERMGFTEVTP VTGASLRRTM LLLSRSPEA

Concentration

0.1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of ADAR in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using a ADAR Polyclonal Antibody (Product # PA5-52014). Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of ADAR in human cell line U-251 MG shows positivity in nucleoli & nucleus but excluded from the nucleoli. Samples were probed using an ADAR Polyclonal Antibody (Product # PA5-52014).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of ADAR in human colon using ADAR Polyclonal Antibody (Product # PA5-52014).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of ADAR in human cerebral cortex, colon, kidney and liver using ADAR Polyclonal Antibody (Product # PA5-52014) (A) shows similar protein distribution across tissues to an independent ADAR Polyclonal Antibody (B).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of ADAR in human liver using ADAR Polyclonal Antibody (Product # PA5-52014).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of ADAR in human kidney using ADAR Polyclonal Antibody (Product # PA5-52014).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of ADAR in human cerebral cortex using ADAR Polyclonal Antibody (Product # PA5-52014).