Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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Validation data
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- Product number
- PA5-47968 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ADAM9 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- In sandwich ELISAs, approximately 20% cross-reactivity with recombinant mouse (rm) rmADAM9 is observed and less than 0.05% cross-reactivity with recombinant human (rh) rhADAM8, rhADAM10, rhTACE, rhTIMP-1, rhTIMP-2, rhTIMP-3, rhTIMP-4, rhBACE-1 and rmADAM10 is observed.
- Concentration
- 0.2 mg/mL
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Knockout validation by Western blot analysis of ADAM9 in lysates of HeLa human cervical epithelial carcinoma parental cell line and ADAM9 knockout HeLa cell line (KO). Samples were incubated in ADAM9 polyclonal antibody (Product # PA5-47968) using a dilution of 1 µg/mL followed by a HRP-conjugated Anti-Sheep IgG secondary antibody. Specific bands were detected for ADAM9 at approximately 78 and 110 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH is shown as a loading control. This experiment was conducted under reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ADAM9 in WI‚38 human lung fibroblast cell line and U‚87 MG human glioblastoma/astrocytoma cell line. Samples were incubated in ADAM9 polyclonal antibody (Product # PA5-47968) using a dilution of 1 µg/mL followed by a HRP-conjugated Anti-Goat IgG secondary antibody. Specific bands were detected for ADAM9 at approximately 110 and 80 kDa (as indicated). This experiment was conducted under reducing conditions.