Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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Validation data
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- Product number
- GTX79189 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX79189, RRID:AB_11164868
- Product name
- PAX6 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat, Sheep, Simian
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- WB analysis of rat eye extract using a PAX6 antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- WB analysis of ovine retinal extract using a PAX6 antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- WB analysis of indicated cell lysates (50ug per lane) using PAX6 antibody at a dilution of 1:1000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ICC/IF analysis of untreated or All-Trans-Retinoic Acid (ATRA) treated (10uM, 24 hours) NCCIT cells using PAX6 antibody at a dilution of 1:50 (green). F-Actin (red) was stained with DyLight 554 Phalloidin and nuclei (blue) were stained with Hoechst 33342. Images were taken at 20X magnification.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- IP analysis was performed on 500ug of 293T extract with 5ug of PAX6 antibody. The precipitated protein was detected by the same antibody at a dilution of 1:1000.