Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- 12-3601-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CD360 (IL-21 Receptor) Monoclonal Antibody (2SX21R), PE, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: This 2SX21R monoclonal antibody reacts with human CD360 (Interleukin-21 Receptor, IL-21R). IL-21R forms a heterodimeric receptor complex with the common gamma chain to form a functional receptor for IL-21. IL-21 is a class I cytokine of the common gamma chain family of cytokines and signals through the IL-21R complex to activate the JAK/STAT (Janus Kinase/Signal Transducers and Activators of Transcription) signaling pathway. Activation of the JAK/STAT pathway by IL-21 promotes transcription of genes responsive to STAT1 and STAT3. IL-21 is mainly produced by CD4+ T cells and mediates the proliferation, differentiation, and effector functions of cells expressing IL-21R such as B cells, T cells, NK cells, dendritic cells and other populations of myeloid cells. IL-21 and its receptor are implicated in the pathogenesis of chronic viral infections, autoimmunity, allergy, organ transplantation, and cancer. Applications Reported: This 2SX21R antibody has been reported for use in flow cytometric analysis. Applications Tested: This 2SX21R antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Yellow dye
- Isotype
- IgG
- Antibody clone number
- 2SX21R
- Vial size
- 25 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Interleukin-21 and T follicular helper cells in HIV infection: research focus and future perspectives.
Crystal structure of interleukin-21 receptor (IL-21R) bound to IL-21 reveals that sugar chain interacting with WSXWS motif is integral part of IL-21R.
TIM-3 is expressed on activated human CD4+ T cells and regulates Th1 and Th17 cytokines.
Interleukin-21: a modulator of lymphoid proliferation, apoptosis and differentiation.
Interleukin-21 and the IL-21 receptor: novel effectors of NK and T cell responses.
Pallikkuth S, Pahwa S
Immunologic research 2013 Dec;57(1-3):279-91
Immunologic research 2013 Dec;57(1-3):279-91
Crystal structure of interleukin-21 receptor (IL-21R) bound to IL-21 reveals that sugar chain interacting with WSXWS motif is integral part of IL-21R.
Hamming OJ, Kang L, Svensson A, Karlsen JL, Rahbek-Nielsen H, Paludan SR, Hjorth SA, Bondensgaard K, Hartmann R
The Journal of biological chemistry 2012 Mar 16;287(12):9454-60
The Journal of biological chemistry 2012 Mar 16;287(12):9454-60
TIM-3 is expressed on activated human CD4+ T cells and regulates Th1 and Th17 cytokines.
Hastings WD, Anderson DE, Kassam N, Koguchi K, Greenfield EA, Kent SC, Zheng XX, Strom TB, Hafler DA, Kuchroo VK
European journal of immunology 2009 Sep;39(9):2492-501
European journal of immunology 2009 Sep;39(9):2492-501
Interleukin-21: a modulator of lymphoid proliferation, apoptosis and differentiation.
Leonard WJ, Spolski R
Nature reviews. Immunology 2005 Sep;5(9):688-98
Nature reviews. Immunology 2005 Sep;5(9):688-98
Interleukin-21 and the IL-21 receptor: novel effectors of NK and T cell responses.
Parrish-Novak J, Foster DC, Holly RD, Clegg CH
Journal of leukocyte biology 2002 Nov;72(5):856-63
Journal of leukocyte biology 2002 Nov;72(5):856-63
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with Anti-Human CD19 PE-Cyanine7 (Product # 25-0198-42) and 0.5 µg of Mouse IgG1 K Isotype Control PE (Product # 12-4714-81) (left) or 0.5 µg of Anti-Human CD360 (IL-21 Receptor) PE (right). Cells in the lymphocyte gate were used for analysis.
- Conjugate
- Yellow dye