Explore
Validate
Learn
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Flow cytometry [1]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 46-3601-42 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD360 (IL-21 Receptor) Monoclonal Antibody (2SX21R), PerCP-eFluor™ 710, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: This 2SX21R monoclonal antibody reacts with human CD360 (Interleukin-21 Receptor, IL-21R). IL-21R forms a heterodimeric receptor complex with the common gamma chain to form a functional receptor for IL-21. IL-21 is a class I cytokine of the common gamma chain family of cytokines and signals through the IL-21R complex to activate the JAK/STAT (Janus Kinase/Signal Transducers and Activators of Transcription) signaling pathway. Activation of the JAK/STAT pathway by IL-21 promotes transcription of genes responsive to STAT1 and STAT3. IL-21 is mainly produced by CD4+ T cells and mediates the proliferation, differentiation, and effector functions of cells expressing IL-21R such as B cells, T cells, NK cells, dendritic cells and other populations of myeloid cells. IL-21 and its receptor are implicated in the pathogenesis of chronic viral infections, autoimmunity, allergy, organ transplantation, and cancer.
- Antibody clone number
- 2SX21R
- Concentration
- 5 µL/Test
Submitted references B cell-intrinsic changes with age do not impact antibody-secreting cell formation but delay B cell participation in the germinal centre reaction.
Lee JL, Fra-Bido SC, Burton AR, Innocentin S, Hill DL, Linterman MA
Aging cell 2022 Sep;21(9):e13692
Aging cell 2022 Sep;21(9):e13692
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with Anti-Human CD19 APC (Product # 17-0198-42) and Mouse IgG1 K Isotype Control PerCP-eFluor® 710 (Product # 46-4714-82) (left) or Anti-Human CD360 (IL-21 Receptor) PerCP-eFluor® 710 (right). Cells in the lymphocyte gate were used for analysis.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 3 FIGURE B cells from older human donors are able to upregulate costimulatory molecules and activation markers after stimulation. (a-e) Representative flow cytometric histograms showing the expression levels of costimulatory molecules by memory B cells from a 29 year old (grey) and 70 year old (blue) donor after 48 h stimulation with CD40L and IL-21 or media (orange) and graphs showing the percentages of cells positive for the marker, or geometric mean of marker using the gating strategies shown, for CD80 (a), CD86 (b), HLA-DR (c), CD69 (d) and IL-21R (e). These were gated on live CD19 + CD20 + cells. Fluorescent minus one (FMO) controls were included for the IL-21R stain and are shown on the representative histogram. Bar height corresponds to the mean, error bars indicate standard deviation, and each symbol represents values from independent donors. Statistics were calculated using the unpaired Mann-Whitney U test. Data were representative of three independent repeat experiments.
