Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- AM05643PU-S - Provider product page
- Provider
- OriGene
- Product name
- IRF3 (108-166) mouse monoclonal antibody, clone 3F10, Purified
- Antibody type
- Monoclonal
- Description
- IRF3 (108-166) mouse monoclonal antibody, clone 3F10, Purified
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- IRF3
- Isotype
- IgG
- Antibody clone number
- 3F10
- Vial size
- 50 µl
- Concentration
- 1.0 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis on HeLa cells: Cell lysates of HeLa (40 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human IRF-3 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescence of human HeLa cells stained with IRF-3 (1:500) with Texas-Red and Hoechst 33342 (Blue) for nucleus staining.
- Validation comment
- IF