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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- AF8524 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal Cadherin-17 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse and human Cadherin-17 in direct ELISAs and Western blots. In direct ELISAs, less then 10% cross-reactivity with recombinant human Cadherin-17 is observed.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 uL
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC, as supplied. 1 month, 2 to 8 degreesC under sterile conditions after opening. 6 months, -20 to -70 degreesC under sterile conditions after opening.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human and Mouse Cadherin-17 by Western Blot. Western blot shows lysates of human colon tissue and mouse colon tissue. PVDF membrane was probed with 1:1000 dilution of Rabbit Anti-Human/Mouse Cadherin-17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8524) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Cadherin-17 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human and mouse Cadherin-17 by Simple WesternTM. Simple Western lane view shows lysates of human colon tissue and mouse colon tissue, loaded at 0.2 mg/mL. Specific bands were detected for Cadherin-17 at approximately 152 and 146 kDa (as indicated) using 1:100 dilution of Rabbit Anti-Human/Mouse Cadherin-17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8524) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Cadherin-17 in Mouse Intestine. Cadherin-17 was detected in perfusion fixed frozen sections of mouse intestine using Rabbit Anti-Mouse Cadherin-17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8524) at 1:250 dilution overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to the plasma membrane of glandular cells. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
