AF5989

antibody from Novus Biologicals

Targeting: IL18 IGIF, IL-18, IL-1g, IL1F4

Western blot (Data presented on provider website)

Blocking/Neutralizing (Supportive data in Antibodypedia)

Antibody Data

Product number

AF5989

Provider

Novus Biologicals

Product name

Sheep Polyclonal IL-18/IL-1F4 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects feline IL-18/IL-1F4 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant canine IL-18, approximately 30% cross-reactivity with recombinant porcine IL-18, approximately 20% cross-reactivity with recombinant human IL-18 and recombinant rat IL-18, and less than 10% cross-reactivity with recombinant mouse IL-18 and recombinant rhesus macaque IL-18 is observed.

Reactivity

Feline

Host

Sheep

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Blocking/Neutralizing

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

IFN-gamma Secretion Induced by IL-18/IL-1F4 and Neutralization by Feline IL-18/IL-1F Antibody. In the presence of Recombinant Human TNF-alpha (Catalog # 210-TA), Recombinant Feline IL-18/IL-1F4 (Catalog # 5989-RM) stimulates IFN-gamma secretion in the KG-1 human acute myelogenous leukemia cell line in a dose-dependent manner (orange line), as measured by the Human IFN-gamma Quantikine ELISA Kit (Catalog # DIF50). Under these conditions, IFN-gamma secretion elicited by Recombinant Feline IL-18/IL-1F4 (1 µg/mL) is neutralized (green line) by increasing concentrations of Sheep Anti-Feline IL-18/IL-1F4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5989). The ND₅₀ is typically 2-12 µg/mL.