orb146772

antibody from Biorbyt

Targeting: HSP90B1 GP96, GRP94, TRA1

Western blot (Recommended by provider)

Immunoprecipitation (Recommended by provider)

Flow cytometry (Recommended by provider)

Antibody Data

Product number

orb146772

Provider

Biorbyt

Product name

GRP94 antibody (PE/ATTO-594)

Antibody type

Monoclonal

Description

Rat monoclonal to GRP94 (PE/ATTO-594). Grp94 (glucose regulated protein 94, gp96) is a constitutively expressed endoplasmic reticulum (ER) lumenal protein that is up-regulated in response to cellular stress such as heat shock, oxidative stress or glucose depletion. Grp94 is thought to play a role in protein translocation to the ER, in their subsequent folding and assembly, and in regulating protein secretion. Grp94 also plays a role in antigen presentation by accessing the endogenous pathway and eliciting specific CTL responses to chaperone bound peptides via MHC class I pathway Grp94 is a member of the Hsp90 family of stress proteins and shares sequence homology with its cytosolic equivalent, Hsp90. Both Hsp90 and Grp94 are calcium binding proteins. Despite sharing 50% sequence homology over its N domains and complete conservation in its ligand binding domains with Hsp90, Grp94 and Hsp90 differ in their interactions with regulatory ligands as Grp94 has weak ATP binding and hydrolysis activity. Grp94 exists as a homodimer and the two subunits interact at two distinct intermolecular sites, C terminal dimerization domains and the N-terminal interacts with the middle domain of opposing subunits. Grp94 contains a carboxy terminal KDEL (Lys-Asp-Glu-Leu) sequence which is believed to aid in its retention in the ER..

Reactivity

Human, Mouse, Guinea Pig, Hamster, Porcine, Rabbit, Sheep, Simian, Xenopus

Host

Rat

Conjugate

Red dye

Isotype

IgG

Antibody clone number

9G10

Vial size

200 μg

Concentration

1 mg/ml

Storage

Store at 2-8°C and -20°C for one year. (Shipped on ice packs)