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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Immunocytochemistry [1]
- Other assay [3]
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- Product number
- PA1-013-A555 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- KDEL Polyclonal Antibody, Alexa Fluor™ 555
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- PA1013A555 detects KDEL from human, rat, mouse and hamster.
- Concentration
- 1 mg/mL
Submitted references Hyaluronan Rafts on Airway Epithelial Cells.
Primary murine airway smooth muscle cells exposed to poly(I,C) or tunicamycin synthesize a leukocyte-adhesive hyaluronan matrix.
Differentiated murine airway epithelial cells synthesize a leukocyte-adhesive hyaluronan matrix in response to endoplasmic reticulum stress.
Abbadi A, Lauer M, Swaidani S, Wang A, Hascall V
The Journal of biological chemistry 2016 Jan 15;291(3):1448-55
The Journal of biological chemistry 2016 Jan 15;291(3):1448-55
Primary murine airway smooth muscle cells exposed to poly(I,C) or tunicamycin synthesize a leukocyte-adhesive hyaluronan matrix.
Lauer ME, Mukhopadhyay D, Fulop C, de la Motte CA, Majors AK, Hascall VC
The Journal of biological chemistry 2009 Feb 20;284(8):5299-312
The Journal of biological chemistry 2009 Feb 20;284(8):5299-312
Differentiated murine airway epithelial cells synthesize a leukocyte-adhesive hyaluronan matrix in response to endoplasmic reticulum stress.
Lauer ME, Erzurum SC, Mukhopadhyay D, Vasanji A, Drazba J, Wang A, Fulop C, Hascall VC
The Journal of biological chemistry 2008 Sep 19;283(38):26283-96
The Journal of biological chemistry 2008 Sep 19;283(38):26283-96
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of KDEL/ER (orange) in HeLa cells. The cells were permeabilized with 0.1% Triton X-100 in TBS for 15 minutes, and blocked with 3% Blocker BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were stained with a KDEL/ER Rabbit polyclonal antibody, AlexaFluor 555 conjugate (Product # PA1-013-A555), at a concentration of 10 µg/mL in blocking buffer for at least 1 hour at room temperature protected from light. Nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249) at a dilution of 1:10,000 in blocking buffer. Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
