Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- MAB8762 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal IGFBP-6 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human IGFBP-6 in direct ELISAs. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse (rm) IGFBP-6 is observed, less than 4% cross-reactivity with rmIGFBP-3 and recombinant human (rh) IGFBP-4 is observed, and no cross-reactivity with rhIGFBP-1, -2, -3, rmIGFBP-1, or -2 is observed.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 500 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- IGFBP-6 Inhibition of IGF-II-dependent Cell Proliferation and Neutralization by Human IGFBP-6 Antibody. Recombinant Human IGFBP-6 (Catalog # 876-B6) inhibits Recombinant Human IGF-II (Catalog # 292-G2) induced proliferation in the MCF-7 human breast cancer cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human IGF-II (14 ng/mL) activity elicited by Recombinant Human IGFBP-6 (0.8 µg/mL) is neutralized (green line) by increasing concen-trations of Mouse Anti-Human IGFBP-6 Monoclonal Antibody (Catalog # MAB8762). The ND50 is typically 5-25 µg/mL.