NBP1-83747

antibody from Novus Biologicals

Targeting: TRIM33 FLJ11429, KIAA1113, PTC7, RFG7, TF1G, TIF1G, TIF1GAMMA, TIFGAMMA

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-83747

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-83747, RRID:AB_11003475

Product name

Rabbit Polyclonal TIF1 gamma Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Specificity of human TIF1 gamma antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: TIF1 gamma Antibody [NBP1-83747] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: TIF1 gamma Antibody [NBP1-83747] - Analysis in human cell lines MCF-7 and HeLa. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: TIF1 gamma Antibody [NBP1-83747] - Staining of human hippocampus shows strong nuclear positivity in neuronal cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: TIF1 gamma Antibody [NBP1-83747] - Staining of human cerebellum shows strong nuclear positivity in cells in granular layer.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: TIF1 gamma Antibody [NBP1-83747] - Staining of human placenta shows strong nuclear positivity in trophoblastic cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: TIF1 gamma Antibody [NBP1-83747] - Staining of human skeletal muscle shows no positivity in myocytes as expected.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: TIF1 gamma Antibody [NBP1-83747] - Staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: TIF1 gamma Antibody [NBP1-83747] - Staining in human placenta and skeletal muscle tissues using NBP1-83747 antibody. Corresponding TRIM33 RNA-seq data are presented for the same tissues.