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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunohistochemistry [2]
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- Product number
- PA5-28299 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NFIB Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: nuclear factor I/B tansfected 293T cell, mouse brain.
- Concentration
- 0.57 mg/mL
Submitted references Tumor Heterogeneity Underlies Differential Cisplatin Sensitivity in Mouse Models of Small-Cell Lung Cancer.
Böttger F, Semenova EA, Song JY, Ferone G, van der Vliet J, Cozijnsen M, Bhaskaran R, Bombardelli L, Piersma SR, Pham TV, Jimenez CR, Berns A
Cell reports 2019 Jun 11;27(11):3345-3358.e4
Cell reports 2019 Jun 11;27(11):3345-3358.e4
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using NFIB Polyclonal Antibody (Product # PA5-28299). Sample (50 µg of whole cell lysate). Lane A: Mouse brain. 7.5% SDS PAGE. NFIB Polyclonal Antibody (Product # PA5-28299) diluted at 1:3,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of NFIB expression in transfected 293T cell line by NFIB polyclonal antibody. Lane A: Non-transfected lysate. Lane B: NFIB transfected lysate. 12% SDS PAGE. NFIB Polyclonal Antibody (Product # PA5-28299) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NFIB Polyclonal Antibody detects NFIB protein at nucleus on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. NFIB Polyclonal Antibody (Product # PA5-28299) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NFIB Polyclonal Antibody detects NFIB protein at nucleus on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. NFIB Polyclonal Antibody (Product # PA5-28299) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
