AF225
antibody from R&D Systems
Targeting: TNFRSF1A
CD120a, TNF-R, TNF-R-I, TNF-R55, TNFAR, TNFR1, TNFR60
Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Immunocytochemistry [1]
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- Product number
- AF225 - Provider product page
- Provider
- R&D Systems
- Product name
- Human TNF RI/TNFRSF1A Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human sTNF RI/TNFRSF1A in direct ELISAs and Western blots. In direct ELISAs, less than 20% cross-reactivity with recombinant mouse TNF sRI and recombinant rat TNF sRI is observed. This anti-human TNF RI antibody can interact with the cell surface 55 kDa human TNF RI and exhibits TNF agonist activities on the A549 human lung carcinoma cell line. This antibody also exhibits TNF agonist activities on the L-929 mouse fibroblast cell line, suggesting that it has cross-species activity with mouse cell surface TNF RI.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
NP_001056
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references NKp46+ Innate Lymphoid Cells Dampen Vaginal CD8 T Cell Responses following Local Immunization with a Cholera Toxin-Based Vaccine.
Ubiquitin-associated domain-containing ubiquitin regulatory X (UBX) protein UBXN1 is a negative regulator of nuclear factor κB (NF-κB) signaling.
Pre-analytical effects of blood sampling and handling in quantitative immunoassays for rheumatoid arthritis.
Inflammatory cytokines stimulate the adhesion of colon carcinoma cells to mesothelial monolayers.
Stent implantation in coronary porcine arteries is associated with early activation of TNFalpha and TNFalpha receptor II expression.
Luci C, Bekri S, Bihl F, Pini J, Bourdely P, Nouhen K, Malgogne A, Walzer T, Braud VM, Anjuère F
PloS one 2015;10(12):e0143224
PloS one 2015;10(12):e0143224
Ubiquitin-associated domain-containing ubiquitin regulatory X (UBX) protein UBXN1 is a negative regulator of nuclear factor κB (NF-κB) signaling.
Wang YB, Tan B, Mu R, Chang Y, Wu M, Tu HQ, Zhang YC, Guo SS, Qin XH, Li T, Li WH, Li AL, Zhang XM, Li HY
The Journal of biological chemistry 2015 Apr 17;290(16):10395-405
The Journal of biological chemistry 2015 Apr 17;290(16):10395-405
Pre-analytical effects of blood sampling and handling in quantitative immunoassays for rheumatoid arthritis.
Zhao X, Qureshi F, Eastman PS, Manning WC, Alexander C, Robinson WH, Hesterberg LK
Journal of immunological methods 2012 Apr 30;378(1-2):72-80
Journal of immunological methods 2012 Apr 30;378(1-2):72-80
Inflammatory cytokines stimulate the adhesion of colon carcinoma cells to mesothelial monolayers.
van Grevenstein WM, Hofland LJ, van Rossen ME, van Koetsveld PM, Jeekel J, van Eijck CH
Digestive diseases and sciences 2007 Oct;52(10):2775-83
Digestive diseases and sciences 2007 Oct;52(10):2775-83
Stent implantation in coronary porcine arteries is associated with early activation of TNFalpha and TNFalpha receptor II expression.
Hadjadj S, Cloutier I, Geoffroy P, Tanguay JF
Atherosclerosis 2007 May;192(1):25-32
Atherosclerosis 2007 May;192(1):25-32
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- TNF RI/TNFRSF1A in Human Peripheral Blood Lymphocytes. TNF RI/TNFRSF1A was detected in immersion fixed human peripheral blood lymphocytes using 5 µg/mL Human TNF RI/TNFRSF1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF225) for 3 hours at room temperature. Cells were stained (red) and counterstained (green). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.