PA5-36674

antibody from Invitrogen Antibodies

Targeting: NFKB2 LYT-10, NF-kB2, p105, p49/p100, p52

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-36674

Provider

Invitrogen Antibodies

Product name

Phospho-NFkB p52 (Ser865) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody detects endogenous protein at a molecular weight of 100 and 120 kDa.

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Phospho-NFkB p52 (Ser865) in Lane 1: NIH-3T3 whole cell lysate (40 µg), Lane 2: NIH-3T3 (PMA, 60 ng/mL, 5 min)whole cell lysate (40 µg), Lane 3: NIH-3T3 (PMA, 60 ng/mL, 15 min)whole cell lysate (40 µg), Lane 4: NIH-3T3 (PMA, 60 ng/mL, 30 min)whole cell lysate (40 µg), Lane 5: NIH-3T3 (EGF, 0.1 ng/mL, 30 min)whole cell lysate (40 µg). Samples were incubated with Phospho-NFkB p52 (Ser865) polyclonal antibody (Product # PA5-36674) at a dilution of 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Phospho-NFkB p52 (Ser865) in Lane 1: HEK293T whole cell lysate, Lane 2: HEK293T treated with UV for 5min then repair for 1 hour whole cell lysate, Lane 3: HEK293T treated with UV for 5min then repair for 4 hours whole cell lysate, Lane 4: HEK293T treated with UV for 5min then repair for 16 hours whole cell lysate. Samples were incubated with Phospho-NFkB p52 (Ser865) polyclonal antibody (Product # PA5-36674) at a dilution of 1:500.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Phospho-NFkB p52 (Ser865) in paraffin-embedded human breast carcinoma tissue. Samples were incubated with Phospho-NFkB p52 (Ser865) polyclonal antibody (Product # PA5-36674) at a dilution of 1:100.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of Phospho-NF-kappa-B2 p100 pSer865 in paraffin-embedded human breast carcinoma using Phospho-NF-kappa-B2 p100 pSer865 polyclonal antibody (Product # PA5-36674) at a dilution of 1:100.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Phospho-NFkB p52 (Ser865) in paraffin-embedded human breast carcinoma tissue. Samples were incubated with Phospho-NFkB p52 (Ser865) polyclonal antibody (Product # PA5-36674) at a dilution of 1:100.