A300-410A

antibody from Invitrogen Antibodies

Targeting: NCOA6 AIB3, ASC2, KIAA0181, NRC, PRIP, RAP250, TRBP

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

A300-410A

Provider

Invitrogen Antibodies

Product name

ASC2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

The recommended shelf life for this product is 1 year from date of receipt.

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human ASC2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ASC2 antibody A300-410A (lot A300-410A-2) used for IP at 6 µg per reaction. ASC2 was also immunoprecipitated by a previous lot of this antibody (lot A300-410A-1) and rabbit anti-ASC2 antibody A300-411A. For blotting immunoprecipitated ASC2, A300-410A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human ASC2 by western blot. Samples: Whole cell lysate (5, 15 and 50 µg) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ASC2 antibody A300-410A (lot A300-410A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human ASC2 by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-ASC2 (Cat. No. A300-410A Lot2) used at a dilution of 1:1,000 ( 1µg/ml). Detection: DAB.