Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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Validation data
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- Product number
- NB100-490 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-490, RRID:AB_2191181
- Product name
- Rabbit Polyclonal GLUT10 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Human GLUT10 protein.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
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- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GLUT10 Antibody [NB100-490] - Cochlea from embryonic mice and mice up to 1 yr old were removed and the pure membrane fraction loaded on 4-20% SDS gel. Following electrophoresis, transfer and blocking, the blot was incubated overnight at 4C with NB100-490 at 1:500 dilution followed by incubation with secondary Ab at 1:3,000 dilution for 1h at room temp. Protein bands were visualized on x-ray film with SuperSignal west femto ECL substrate. Lane 1:10ug of protein from liver Lane 2:10ug of protein from cochlea (E18) Lane 3:10ug of protein from cochlea (P7) Lane 4:10ug of protein from cochlea (P10) Lane 5:10ug of protein from cochlea (P14) Lane 6:10ug of protein from cochlea (P17) Lane 7:10ug of protein from cochlea (P60) Lane 8:10ug of protein from cochlea (1 Year)
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GLUT10 Antibody [NB100-490] - NCI-H1299 non-small cell lung tumor cells were transfected with the eukaryotic expression vectors pcDNA3.1/V5 (lanes 1,2), pCMV-GLUT10 (lanes 3,4), and pCMV-GLUT1 (lanes 5,6). GLUT10 was detetced using NB100-490.