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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- PA5-93241 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- POLD2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: LQPEEKCCVV GTLFKAMPLQ PSILREVSEE HNLLPQPPRS KYIHPDDELV LEDELQRIKL KGTIDVSKLV TGTVLAVFGS VRDDGKFLVE DYCFADLAPQ KPAPPLDTDR FVLLVSGLGL GGGGGESLLG TQLLVDVVTG QLGDEGEQCS AAHVSRVILA GNLLSHSTQS RDSINKAKYL TKKTQAASVE AVKMLDEILL QLSASVPVDV MPGEFDPTNY TLPQQPLHPC MFPLATAYST L; Positive Samples: HeLa, HL-60, HepG2, BT-474, NCI-H460, Mouse testis; Cellular Location: Nucleus
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.46 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Western Blot analysis of POLD2 in extracts of various cell lines using POLD2 Polyclonal Antibody (Product # PA5-93241) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
