Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- NB300-731AF647 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal GR/NR3C1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified. Using enzymatic digestion analysis, has been shown to react with the undigested 97 kDa GR, a 17 kDa DNA-binding trypsin fragment, and a 45 kDa steroid- and DNA-binding chymotrypsin fragment.
- Reactivity
- Human, Mouse, Rat, Guinea Pig, Rabbit, Sheep, Yeast
- Host
- Mouse
- Conjugate
- Red dye
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C in the dark.
Submitted references In utero Exposure to Maternal Chronic Inflammation Transfers a Pro-Inflammatory Profile to Generation F2 via Sex-Specific Mechanisms.
Chronic Gestational Inflammation: Transfer of Maternal Adaptation over Two Generations of Progeny.
Adams RCM, Smith C
Frontiers in immunology 2020;11:48
Frontiers in immunology 2020;11:48
Chronic Gestational Inflammation: Transfer of Maternal Adaptation over Two Generations of Progeny.
Adams RCM, Smith C
Mediators of inflammation 2019;2019:9160941
Mediators of inflammation 2019;2019:9160941
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
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- Experimental details
- Flow Cytometry: GR/NR3C1 Antibody (BuGR2) [Alexa Fluor 647] [NB300-731AF647] - An intracellular stain was performed on HeLa cells with GR/NR3C1 (BuGR2) antibody NB300-731AF647 (blue) and a matched isotype control NB600-986AF647 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.