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LearnMAB452-100
antibody from Novus Biologicals
Targeting: CXCL2
CINC-2a, GRO2, GROb, MGSA-b, MIP-2a, SCYB2
Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- MAB452-100 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rat Monoclonal CXCL2/GRO beta/MIP-2/CINC-3 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse CXCL2 in ELISAs and Western blots. In ELISAs, does not cross-react with recombinant human (rh) CXCL2/GRO beta , rhCXCL3/GRO gamma , or recombinant mouse CXCL1/KC.
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
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Supportive validation
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- Novus Biologicals (provider)
- Main image
- Experimental details
- Chemotaxis Induced by CXCL2/MIP-2 and Neutralization by Mouse CXCL2/MIP-2 Antibody. Recombinant Mouse CXCL2/MIP-2 (Catalog # 452-M2) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL2/MIP-2 (2 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CXCL2/MIP-2 Monoclonal Antibody (Catalog # MAB452). The ND50 is typically 1-4 µg/mL.
