TA319457

antibody from OriGene

Targeting: EIF3E eIF3-p48, EIF3S6, INT6

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

TA319457

Provider

OriGene

Product name

Rabbit polyclonal Eif3S6 Int6 antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal Eif3S6 Int6 antibody

Reactivity

Canine

Host

Rabbit

Conjugate

Unconjugated

Epitope

Eif3e

Isotype

IgG

Antibody clone number

NULL

Vial size

100 µg

Concentration

0.8 mg/mL

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

WB using Anti-eIF3S6/Int6 antibody shows detection of endogenous eIF3S6/Int6 in whole cell extracts from murine (HC-11 and NIH3T3), monkey (CV-1 and Cos-1), and human (HEK293T) cell lines as well as over-expressed eIF3S6/Int6 (control transfected flag-tagged Int6).The identity of the higher and lower molecular weight bands is unknown. The band at ~48 kDa, indicated by the arrowhead, corresponds to flag-tagged EIF3S6/Int6. Primary antibody was used at 1:1000.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot using affinity purified anti-eIF3S6/Int6 antibody shows detection of endogenous eIF3S6/Int6. Specific staining is not present in lysates containing lentiviral knockdown vectors (shRNA #1 and #2. Control vectors, specifically a scrambled sequence (Ctl NS) and a sequence against an unrelated gene (Ctl #2), were also used. Personal communication, J.Lee, NCI, Bethesda, MD.

Validation comment

WB