AP09387PU-S

antibody from Acris Antibodies GmbH

Targeting: EIF3E eIF3-p48, EIF3S6, INT6

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Antibody Data

Product number

AP09387PU-S

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AP09387PU-S, RRID:AB_2035452

Product name

anti EIF3E / EIF3S6

Antibody type

Polyclonal

Antigen

Synthetic peptide corresponding to a region near the C-terminus of mouse EIF3S6/Int6

Reactivity

Human, Mouse, Rat, Bovine, Canine, Simian

Host

Rabbit

Isotype

IgG

Vial size

25 µl

Concentration

0.8 mg/ml (by UV absorbance at 280 nm)

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Western blot using Affinity purified anti- eIF3S6/Int6 antibody shows detection of endogenous eIF3S6/Int6 in whole cell extracts from Murine (HC-11 and NIH3T3), Monkey (CV-1 and Cos-1), and Human (HEK293T) cell lines as well as over-expressed eIF3S6/Int6 (Control transfected flag-tagged Int6). The identity of the higher and lower molecular weight bands is unknown. The band at ~48 kDa, indicated by the arrowhead, corresponds to flag-tagged EIF3S6/Int6. Primary antibody was used at 1:1000. Personal communication, J.Lee, NCI, Bethesda, MD.

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Western blot using Affinity purified anti- eIF3S6/Int6 antibody shows detection of endogenous eIF3S6/Int6. Specific staining is not present in lysates containing lentiviral knockdown vectors (shRNA #1 and #2. Control vectors, specifically a scrambled sequence (Ctl NS) and a sequence against an unrelated gene (Ctl #2), were also used. Personal communication, J.Lee, NCI, Bethesda, MD.