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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [1]
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- Product number
- AF6676 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Sheep Polyclonal CD4 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects cotton rat CD4 in direct ELISAs. In direct ELISAs, approximately 15% cross-reactivity with recombinant mouse CD4, approximately 5% cross-reactivity with recombinant human CD4, and less than 1% cross-reactivity with recombinant canine CD4 and recombinant feline CD4 is observed.
- Host
- Sheep
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Detection of CD4 in Cotton Rat Splenocytes by Flow Cytometry. Cotton rat splenocytes were stained with Sheep Anti-Cotton Rat CD4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6676, filled histogram) or isotype control antibody (Catalog # 5-001-A, open histogram), followed by Allophycocyanin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0127).
