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- Antibody Data
- Antigen structure
- References [12]
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- Validations
- Flow cytometry [1]
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- Product number
- MHCD0406 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD4 Monoclonal Antibody (S3.5), TRI-COLOR™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- The TRI-COLOR® conjugate is a tandem R-phycoerythrin (PE)-Cy5® conjugate that permits simultaneous multicolor labeling and detection of multiple targets with excitation by a single excitation source-the 488 nm line of the argon-ion laser.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- S3.5
- Vial size
- 500 µL
- Storage
- 4° C, store in dark
Submitted references Ultra-High-Frequency Reprogramming of Individual Long-Term Hematopoietic Stem Cells Yields Low Somatic Variant Induced Pluripotent Stem Cells.
Intermediate DNA methylation is a conserved signature of genome regulation.
Changes in the proportions of CD4(+)T cell subsets defined by CD127 and CD25 expression during HBV infection.
Cytokine signatures of innate and adaptive immunity in 17DD yellow fever vaccinated children and its association with the level of neutralizing antibody.
Induction of allopeptide-specific human CD4+CD25+ regulatory T cells ex vivo.
Expression of CD4 on human peripheral blood neutrophils.
Human CD4+CD25+ regulatory cells have marked and sustained effects on CD8+ T cell activation.
Reduced cell surface expression of CCR5 in CCR5Delta 32 heterozygotes is mediated by gene dosage, rather than by receptor sequestration.
A membrane-proximal basic domain and cysteine cluster in the C-terminal tail of CCR5 constitute a bipartite motif critical for cell surface expression.
Expression and coreceptor activity of STRL33/Bonzo on primary peripheral blood lymphocytes.
Analysis of T-cell clones in systemic lupus erythematosus.
Retrovirally transduced CD34++ human cord blood cells generate T cells expressing high levels of the retroviral encoded green fluorescent protein marker in vitro.
Wang K, Guzman AK, Yan Z, Zhang S, Hu MY, Hamaneh MB, Yu YK, Tolu S, Zhang J, Kanavy HE, Ye K, Bartholdy B, Bouhassira EE
Cell reports 2019 Mar 5;26(10):2580-2592.e7
Cell reports 2019 Mar 5;26(10):2580-2592.e7
Intermediate DNA methylation is a conserved signature of genome regulation.
Elliott G, Hong C, Xing X, Zhou X, Li D, Coarfa C, Bell RJ, Maire CL, Ligon KL, Sigaroudinia M, Gascard P, Tlsty TD, Harris RA, Schalkwyk LC, Bilenky M, Mill J, Farnham PJ, Kellis M, Marra MA, Milosavljevic A, Hirst M, Stormo GD, Wang T, Costello JF
Nature communications 2015 Feb 18;6:6363
Nature communications 2015 Feb 18;6:6363
Changes in the proportions of CD4(+)T cell subsets defined by CD127 and CD25 expression during HBV infection.
Xu HT, Ye J, Chen YB, Zhang LX, Huang JX, Xian JC, Liu L, Peng HL, Li L, Lin M, Huang JH
Immunological investigations 2012;41(3):290-303
Immunological investigations 2012;41(3):290-303
Cytokine signatures of innate and adaptive immunity in 17DD yellow fever vaccinated children and its association with the level of neutralizing antibody.
Luiza-Silva M, Campi-Azevedo AC, Batista MA, Martins MA, Avelar RS, da Silveira Lemos D, Bastos Camacho LA, de Menezes Martins R, de Lourdes de Sousa Maia M, Guedes Farias RH, da Silva Freire M, Galler R, Homma A, Leite Ribeiro JG, Campos Lemos JA, Auxiliadora-Martins M, Eloi-Santos SM, Teixeira-Carvalho A, Martins-Filho OA
The Journal of infectious diseases 2011 Sep 15;204(6):873-83
The Journal of infectious diseases 2011 Sep 15;204(6):873-83
Induction of allopeptide-specific human CD4+CD25+ regulatory T cells ex vivo.
Jiang S, Camara N, Lombardi G, Lechler RI
Blood 2003 Sep 15;102(6):2180-6
Blood 2003 Sep 15;102(6):2180-6
Expression of CD4 on human peripheral blood neutrophils.
Biswas P, Mantelli B, Sica A, Malnati M, Panzeri C, Saccani A, Hasson H, Vecchi A, Saniabadi A, Lusso P, Lazzarin A, Beretta A
Blood 2003 Jun 1;101(11):4452-6
Blood 2003 Jun 1;101(11):4452-6
Human CD4+CD25+ regulatory cells have marked and sustained effects on CD8+ T cell activation.
Câmara NO, Sebille F, Lechler RI
European journal of immunology 2003 Dec;33(12):3473-83
European journal of immunology 2003 Dec;33(12):3473-83
Reduced cell surface expression of CCR5 in CCR5Delta 32 heterozygotes is mediated by gene dosage, rather than by receptor sequestration.
Venkatesan S, Petrovic A, Van Ryk DI, Locati M, Weissman D, Murphy PM
The Journal of biological chemistry 2002 Jan 18;277(3):2287-301
The Journal of biological chemistry 2002 Jan 18;277(3):2287-301
A membrane-proximal basic domain and cysteine cluster in the C-terminal tail of CCR5 constitute a bipartite motif critical for cell surface expression.
Venkatesan S, Petrovic A, Locati M, Kim YO, Weissman D, Murphy PM
The Journal of biological chemistry 2001 Oct 26;276(43):40133-45
The Journal of biological chemistry 2001 Oct 26;276(43):40133-45
Expression and coreceptor activity of STRL33/Bonzo on primary peripheral blood lymphocytes.
Sharron M, Pöhlmann S, Price K, Lolis E, Tsang M, Kirchhoff F, Doms RW, Lee B
Blood 2000 Jul 1;96(1):41-9
Blood 2000 Jul 1;96(1):41-9
Analysis of T-cell clones in systemic lupus erythematosus.
Converso M, Bertero MT, Vallario A, Caligaris-Cappio F
Haematologica 2000 Feb;85(2):118-23
Haematologica 2000 Feb;85(2):118-23
Retrovirally transduced CD34++ human cord blood cells generate T cells expressing high levels of the retroviral encoded green fluorescent protein marker in vitro.
Verhasselt B, De Smedt M, Verhelst R, Naessens E, Plum J
Blood 1998 Jan 15;91(2):431-40
Blood 1998 Jan 15;91(2):431-40
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human peripheral blood lymphocytes were stained using TRI-COLOR® of anti-human CD4 monoclonal antibody (clone S3.5). The negative control profiles represent unstained cells.
