Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- NBP2-52681-0.2mg - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Monoclonal CD4 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified. The antibody binds to human CD4 with a Kd of 1.0 nM and an ED50 of 0.2 ug/ml.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.2 mg
- Storage
- Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CD4 Antibody (CE9.1 (Clenoliximab)) [NBP2-52681] - Human thyroid tumour samples were resolved on a 15% SDS PAGE gel and blots probed with the human IgG4-chimeric version of CE9.1 (NBP2-75910) at 1.5 ug/ml before detection using an anti-human IgG4 secondary antibody. Protein was detected by chemiluminescence. The expected unmodified running size for CD4 is 51kDa (arrowhead). Here some non-specific bands can also be seen. NBP2-75910 successfully detected CD4 in human thyroid tissue samples.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: CD4 Antibody (CE9.1 (Clenoliximab)) [NBP2-52681] - Anti-CD4 staining of formaldehyde fixed paraffin embedded guinea pig skin tissue, at 40x magnification. The human IgG4-chimeric version of CE9.1 (Clenoliximab) (NBP2-75910) was used to stain samples at a concentration of 3 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: CD4 Antibody (CE9.1 (Clenoliximab)) [NBP2-52681] - Flow-cytometry using the anti-CD4 research biosimilar antibody Clenoliximab (NBP2-52681) Human lymphocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of Clenoliximab (panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.