Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Flow cytometry [3]
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Validation data
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- Product number
- NBP2-52663-0.2mg - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Monoclonal CD4 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified. mt310 binds to the extracellular domain D1 of CD4 -the same site as the HIV-1 gp120-binding site
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.2 mg
- Storage
- Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: CD4 Antibody (MT310) [NBP2-52663] - Immunohistochemical staining of guinea pig skin tissue using anti-CD4 antibody. MT310 Anti-CD4 staining of formaldehyde fixed paraffin embedded guinea pig skin tissue, at 40x magnification. A mouse IgG1-chimeric version of MT310 was used to stain samples at a concentration of 3 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: CD4 Antibody (MT310) [NBP2-52663] - Flow-cytometry using anti-CD4 antibody MT310 (NBP2-52663) Human lymphocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of MT310 (panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: CD4 Antibody (MT310) [NBP2-52663] - Flow-cytometry using anti-CD4 antibody MT310 (NBP2-52663) Rhesus monkey lymphocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of MT310 (panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: CD4 Antibody (MT310) [NBP2-52663] - Flow-cytometry using anti-CD4 antibody MT310 (NBP2-52663) Cynomolgus monkey lymphocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of MT310 (panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.