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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [9]
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- Validations
- Flow cytometry [1]
- Other assay [4]
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- Product number
- 11-0047-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD4 Monoclonal Antibody (SK3 (SK-3)), FITC, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The SK3 monoclonal antibody reacts with human CD4, a 59-kDa cell surface receptor expressed by a majority of thymocytes, a subpopulation of mature T helper cells, and, in low levels, monocytes. CD4 is a receptor for the human immunodeficiency virus (HIV). SK3 blocks HIV binding and mixed lymphocyte reaction. The SK3 and RPA-T4 monoclonal antibodies do not cross-block binding, suggesting recognition of distinct epitopes.
- Conjugate
- Green dye
- Antibody clone number
- SK3 (SK-3)
- Concentration
- 5 µL/Test
Submitted references Single-cell multi-omics analysis presents the landscape of peripheral blood T-cell subsets in human chronic prostatitis/chronic pelvic pain syndrome.
Human P2Y(11) Expression Level Affects Human P2X7 Receptor-Mediated Cell Death.
The Genetic Polymorphisms of NLRP3 Inflammasome Associated with T Helper Cells in Patients with Multiple Myeloma.
Characteristic patterns of HLA presentation and T cell differentiation in adult-onset Still's disease.
Chloroquine inhibits human CD4(+) T-cell activation by AP-1 signaling modulation.
HDAC inhibition potentiates immunotherapy in triple negative breast cancer.
Effects of transcutaneous acupoint electrical stimulation on the imbalance of Th(1), Th(2), Th(17) and T(reg) cells following thoracotomy of patients with lung cancer.
CIB1 and CIB2 are HIV-1 helper factors involved in viral entry.
Alloantigen-specific regulatory T cells generated with a chimeric antigen receptor.
Zhang M, Liu Y, Chen J, Chen L, Meng J, Yang C, Yin S, Zhang X, Zhang L, Hao Z, Chen X, Liang C
Journal of cellular and molecular medicine 2020 Dec;24(23):14099-14109
Journal of cellular and molecular medicine 2020 Dec;24(23):14099-14109
Human P2Y(11) Expression Level Affects Human P2X7 Receptor-Mediated Cell Death.
Dreisig K, Sund L, Dommer MW, Kristensen NP, Boddum K, Viste R, Fredholm S, Odum N, Jäättelä M, Skov S, Kornum BR
Frontiers in immunology 2018;9:1159
Frontiers in immunology 2018;9:1159
The Genetic Polymorphisms of NLRP3 Inflammasome Associated with T Helper Cells in Patients with Multiple Myeloma.
Zhao X, Hua M, Yan S, Yu J, Han F, Zhong C, Wang R, Zhang C, Hou M, Ma D
Journal of immunology research 2018;2018:7569809
Journal of immunology research 2018;2018:7569809
Characteristic patterns of HLA presentation and T cell differentiation in adult-onset Still's disease.
Jung JY, Choi B, Sayeed HM, Suh CH, Kim YW, Kim HA, Sohn S
International journal of immunopathology and pharmacology 2018 Jan-Dec;32:2058738418791284
International journal of immunopathology and pharmacology 2018 Jan-Dec;32:2058738418791284
Chloroquine inhibits human CD4(+) T-cell activation by AP-1 signaling modulation.
Schmidt RL, Jutz S, Goldhahn K, Witzeneder N, Gerner MC, Trapin D, Greiner G, Hoermann G, Steiner G, Pickl WF, Burgmann H, Steinberger P, Ratzinger F, Schmetterer KG
Scientific reports 2017 Feb 7;7:42191
Scientific reports 2017 Feb 7;7:42191
HDAC inhibition potentiates immunotherapy in triple negative breast cancer.
Terranova-Barberio M, Thomas S, Ali N, Pawlowska N, Park J, Krings G, Rosenblum MD, Budillon A, Munster PN
Oncotarget 2017 Dec 26;8(69):114156-114172
Oncotarget 2017 Dec 26;8(69):114156-114172
Effects of transcutaneous acupoint electrical stimulation on the imbalance of Th(1), Th(2), Th(17) and T(reg) cells following thoracotomy of patients with lung cancer.
Wu H, Wang K, Li G, Meng D, Han J, Wang G, Li YU
Experimental and therapeutic medicine 2016 Feb;11(2):495-502
Experimental and therapeutic medicine 2016 Feb;11(2):495-502
CIB1 and CIB2 are HIV-1 helper factors involved in viral entry.
Godinho-Santos A, Hance AJ, Gonçalves J, Mammano F
Scientific reports 2016 Aug 4;6:30927
Scientific reports 2016 Aug 4;6:30927
Alloantigen-specific regulatory T cells generated with a chimeric antigen receptor.
MacDonald KG, Hoeppli RE, Huang Q, Gillies J, Luciani DS, Orban PC, Broady R, Levings MK
The Journal of clinical investigation 2016 Apr 1;126(4):1413-24
The Journal of clinical investigation 2016 Apr 1;126(4):1413-24
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with Anti-Human CD8a APC (Product # 17-0088-42) and Mouse IgG1 K Isotype Control FITC (Product # 11-4714-42) (left) or Anti-Human CD4 FITC (right). Cells in the lymphocyte gate were used for analysis.
- Conjugate
- Green dye
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Conjugate
- Green dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Conjugate
- Green dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Supplement Figure 2. Representative examples of flow cytometric dot plots of surface-stained cells presenting CD4+CCR7+, CD8+CCR7+, CD4+CD62L-, and CD8+CD62L- from the peripheral blood of one patient with adult-onset Still's disease (AOSD), a patient with rheumatoid arthritis (RA), and a healthy control (HC).
- Conjugate
- Green dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 9 Effect of downmodulation of CD4 and CIB proteins in CD4+ T-lymphocytes on the expression of proteins implicated in HIV-1 entry. Activated CD4+ T-lymphocytes were transduced with the empty vector (pLKO.1) or vectors leading to the expression of the indicated shRNAs, and the surface expression of the following molecules was measured by flow cytometry: CD3, CD4, CXCR4, LFA-1 and alpha4beta7. Intracellular talin expression was also evaluated in permeabilized cells. In each case, the mean fluorescence intensity (MFI) and a representative fluorescence histogram are shown. For MFI, the values represent the mean +- SEM of 4 independent experiments using two different shRNAs per targeted gene and are expressed relative to values obtained for non-transduced cells. *P < 0.05, **P < 0.01 versus sh-SCRAM (Kruskal-Wallis test).
- Conjugate
- Green dye
