Antibody data
- Antibody Data
- Antigen structure
- References [7]
- Comments [0]
- Validations
- Flow cytometry [1]
- Other assay [4]
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- Product number
- 12-0047-42 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD4 Monoclonal Antibody (SK3 (SK-3)), PE, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The SK3 monoclonal antibody reacts with human CD4, a 59-kDa cell surface receptor expressed by a majority of thymocytes, a subpopulation of mature T helper cells, and, in low levels, monocytes. CD4 is a receptor for the human immunodeficiency virus (HIV). SK3 blocks HIV binding and mixed lymphocyte reaction. The SK3 and RPA-T4 monoclonal antibodies do not cross-block binding, suggesting recognition of distinct epitopes.
- Conjugate
- Yellow dye
- Antibody clone number
- SK3 (SK-3)
- Concentration
- 5 µL/Test
Submitted references Regulatory T cell features in chronic granulomatous disease.
Extracellular vesicles from human saliva promote hemostasis by delivering coagulant tissue factor to activated platelets.
Characteristic patterns of HLA presentation and T cell differentiation in adult-onset Still's disease.
HDAC inhibition potentiates immunotherapy in triple negative breast cancer.
Promoter H3K4 methylation dynamically reinforces activation-induced pathways in human CD4 T cells.
CIB1 and CIB2 are HIV-1 helper factors involved in viral entry.
Alloantigen-specific regulatory T cells generated with a chimeric antigen receptor.
van de Geer A, Cuadrado E, Slot MC, van Bruggen R, Amsen D, Kuijpers TW
Clinical and experimental immunology 2019 Aug;197(2):222-229
Clinical and experimental immunology 2019 Aug;197(2):222-229
Extracellular vesicles from human saliva promote hemostasis by delivering coagulant tissue factor to activated platelets.
Yu Y, Gool E, Berckmans RJ, Coumans FAW, Barendrecht AD, Maas C, van der Wel NN, Altevogt P, Sturk A, Nieuwland R
Journal of thrombosis and haemostasis : JTH 2018 Jun;16(6):1153-1163
Journal of thrombosis and haemostasis : JTH 2018 Jun;16(6):1153-1163
Characteristic patterns of HLA presentation and T cell differentiation in adult-onset Still's disease.
Jung JY, Choi B, Sayeed HM, Suh CH, Kim YW, Kim HA, Sohn S
International journal of immunopathology and pharmacology 2018 Jan-Dec;32:2058738418791284
International journal of immunopathology and pharmacology 2018 Jan-Dec;32:2058738418791284
HDAC inhibition potentiates immunotherapy in triple negative breast cancer.
Terranova-Barberio M, Thomas S, Ali N, Pawlowska N, Park J, Krings G, Rosenblum MD, Budillon A, Munster PN
Oncotarget 2017 Dec 26;8(69):114156-114172
Oncotarget 2017 Dec 26;8(69):114156-114172
Promoter H3K4 methylation dynamically reinforces activation-induced pathways in human CD4 T cells.
LaMere SA, Thompson RC, Komori HK, Mark A, Salomon DR
Genes and immunity 2016 Jul;17(5):283-97
Genes and immunity 2016 Jul;17(5):283-97
CIB1 and CIB2 are HIV-1 helper factors involved in viral entry.
Godinho-Santos A, Hance AJ, Gonçalves J, Mammano F
Scientific reports 2016 Aug 4;6:30927
Scientific reports 2016 Aug 4;6:30927
Alloantigen-specific regulatory T cells generated with a chimeric antigen receptor.
MacDonald KG, Hoeppli RE, Huang Q, Gillies J, Luciani DS, Orban PC, Broady R, Levings MK
The Journal of clinical investigation 2016 Apr 1;126(4):1413-24
The Journal of clinical investigation 2016 Apr 1;126(4):1413-24
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with Anti-Human CD3 APC (Product # 17-0038-42) and Mouse IgG1 K Isotype Control PE (Product # 12-4714-81) (left) or Anti-Human CD4 PE (Product # 12-0047-42) (right). Cells in the lymphocyte gate were used for analysis.
- Conjugate
- Yellow dye
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Conjugate
- Yellow dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Conjugate
- Yellow dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Supplement Figure 2. Representative examples of flow cytometric dot plots of surface-stained cells presenting CD4+CCR7+, CD8+CCR7+, CD4+CD62L-, and CD8+CD62L- from the peripheral blood of one patient with adult-onset Still's disease (AOSD), a patient with rheumatoid arthritis (RA), and a healthy control (HC).
- Conjugate
- Yellow dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 9 Effect of downmodulation of CD4 and CIB proteins in CD4+ T-lymphocytes on the expression of proteins implicated in HIV-1 entry. Activated CD4+ T-lymphocytes were transduced with the empty vector (pLKO.1) or vectors leading to the expression of the indicated shRNAs, and the surface expression of the following molecules was measured by flow cytometry: CD3, CD4, CXCR4, LFA-1 and alpha4beta7. Intracellular talin expression was also evaluated in permeabilized cells. In each case, the mean fluorescence intensity (MFI) and a representative fluorescence histogram are shown. For MFI, the values represent the mean +- SEM of 4 independent experiments using two different shRNAs per targeted gene and are expressed relative to values obtained for non-transduced cells. *P < 0.05, **P < 0.01 versus sh-SCRAM (Kruskal-Wallis test).
- Conjugate
- Yellow dye