41-2444-82

antibody from Invitrogen Antibodies

Targeting: CD4

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

41-2444-82

Provider

Invitrogen Antibodies

Product name

CD4 Monoclonal Antibody (N1UG0), eFluor™ 570, eBioscience™

Antibody type

Monoclonal

Antigen

Other

Description

Description: The N1UG0 monoclonal antibody reacts with human CD4, a 59 kDa cell surface glycoprotein expressed by the majority of thymocytes, a subpopulation of mature T cells (T-helper cells) and in low levels on monocytes. CD4 is a receptor for the human immunodeficiency virus (HIV). The N1UG0 antibody is recommended for use in staining human formalin-fixed paraffin embedded tissue sections.

Antibody clone number

N1UG0

Concentration

0.2 mg/mL

References

Submitted references

Molecular Mechanism of Sphingosine-1-Phosphate Receptor 1 Regulating CD4(+) Tissue Memory in situ T Cells in Primary Sjogren's Syndrome.

Yang XX, Yang C, Wang L, Zhou YB, Yuan X, Xiang N, Wang YP, Li XM
International journal of general medicine 2021;14:6177-6188

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CD161 contributes to prenatal immune suppression of IFNγ-producing PLZF+ T cells.

Halkias J, Rackaityte E, Hillman SL, Aran D, Mendoza VF, Marshall LR, MacKenzie TC, Burt TD
The Journal of clinical investigation 2019 May 30;129(9):3562-3577

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Highly multiplexed immunofluorescence images and single-cell data of immune markers in tonsil and lung cancer.

Rashid R, Gaglia G, Chen YA, Lin JR, Du Z, Maliga Z, Schapiro D, Yapp C, Muhlich J, Sokolov A, Sorger P, Santagata S
Scientific data 2019 Dec 17;6(1):323

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Highly multiplexed immunofluorescence imaging of human tissues and tumors using t-CyCIF and conventional optical microscopes.

Lin JR, Izar B, Wang S, Yapp C, Mei S, Shah PM, Santagata S, Sorger PK
eLife 2018 Jul 11;7

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Inhibition of gp160 and CD4 maturation in U937 cells after both defective and productive infections by human immunodeficiency virus type 1.

Bour S, Boulerice F, Wainberg MA
Journal of virology 1991 Dec;65(12):6387-96

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of formalin-fixed paraffin embedded human tonsil using 20 µg/mL of Anti-Human CD4 eFluor® 570. Nuclei are stained with DAPI.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 1 S1PR1 expression in CD4 + CD69 + T RM cells. ( A ) Lymphocyte infiltration was detected by H&E staining of the labial salivary glands(LSG) of patients with pSS. ( B ) Expression of S1PR1 was detected by immunohistochemistry in labial salivary glands (LSG) of patients with pSS. ( C ) Immunofluorescence was performed to detect the expression of CD4, CD69 and S1PR1 in LSG samples with lymphocyte infiltration foci. Bar=20mum. DAPI was used to stain the nuclei and glowed blue. CD4 was pink light, CD69 was red light, and S1PR1 was green light. The yellow arrows point to positive fluorescence results and the white arrows point to negative fluorescence results in the DAPI, CD4, CD69 and S1PR1 image. In the in Merge image, the yellow arrows represent only S1PR1 expression in CD4 + T cells and no surface expression of CD69, the white arrows point to the CD4 + CD69 + T cells without the expression of S1PR1.