MAB6240

antibody from Novus Biologicals

Targeting: ACP3 ACP-3, ACPP, PAP, TM-PAP

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB6240

Provider

Novus Biologicals

Product name

Mouse Monoclonal Prostatic Acid Phosphatase/ACPP Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human Prostatic Acid Phosphatase/ACPP in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human TRACP/PAP/ACP5 is observed.

Reactivity

Human

Host

Mouse

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human Prostatic Acid Phosphatase/ACPP by Western Blot. Western blot shows lysates of human prostate tissue. PVDF Membrane was probed with 2 µg/mL of Human Prostatic Acid Phosphatase/ACPP Monoclonal Antibody (Catalog # MAB6240) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Prostatic Acid Phosphatase/ACPP at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Prostatic Acid Phosphatase/ACPP in Human Prostate. Prostatic Acid Phosphatase/ACPP was detected in immersion fixed paraffin-embedded sections of human prostate using Human Prostatic Acid Phosphatase/ACPP Monoclonal Antibody (Catalog # MAB6240) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hemotoxylin (blue). Specific staining was localized to the cytoplasm of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.