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Immunocytochemistry
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [1]
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Validation data
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- Product number
- AP31817PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP31817PU-N, RRID:AB_11143474
- Product name
- anti ACPP / Prostatic acid phosphatase (Pain System Marker)
- Antibody type
- Polyclonal
- Antigen
- Recombinant Mouse PAP protein was expressed using a baculoviral-delivery system.Preparation: After repeated injections, immune eggs were collected from laying hens, from which IgY antibody were prepared (âanti-PAP IgY fractionâ). Some of this antibody was further purified using an agarose matrix to which the PAP protein was convalently attached (âAffinity-purified anti-PAPâ). The final preparation in the accompanying vial contains 10 mg/ml of the âanti-PAP IgY fractionâ supplemented with 20 mg/ml of the âaffinity-purified anti-PAPâ plus 50% (v/v) Glycerol (to prevent freezing at â20°C). Finally, this antibody preparation was filter-sterilized (0.45 mm) and 200 µl aliquots prepared.
- Reactivity
- Mouse
- Host
- Chicken/Avian
- Vial size
- 0.2 ml
- Concentration
- 10 mg IgY/ml (based on absorbance at 280 nm)
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Supportive validation
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- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Tissue section through an adult Mouse brain showing PAP (red fluorescence) in the superficial laminae of the adult Mouse spinal cord dorsal horn gray matter.
