12-9159-42

antibody from Invitrogen Antibodies

Targeting: DSG2 CDHF5

Provider product page for 12-9159-42

Flow cytometry

Other assay

Antibody data

Antibody Data
Antigen structure
References [4]
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Validations
Flow cytometry [1]
Other assay [4]

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Product number: 12-9159-42 - Provider product page
Provider: Invitrogen Antibodies
Product name: Desmoglein 2 Monoclonal Antibody (CSTEM28), PE, eBioscience™
Antibody type: Monoclonal
Antigen: Other
Description: This monoclonal antibody CSTEM28 reacts with human desmoglein 2 (DSG2), a type 1 transmembrane protein belonging to the cadherin family. DSG2 is expressed by epithelial cells, cardiomyocytes, endothelial progenitors, and CD34+CD45dim hematopoietic stem and progenitor cells. This adhesion molecule forms cell-cell adhesion complexes called desmosomes that provide tensile strength to tissues that experience mechanical stress. Other than its role in desmosome formation, DSG2 plays a role in barrier integrity, adhesion, migration, cell apoptosis, proliferation, vasculogenic mimicry, and intracellular signaling. DSG2-expressing endothelial progenitor cells are pro-angiogenic, suggesting a role in vasculature formation. On hematopoietic stem and progenitor cells, DSG2 expression is highest on CD38-CD90+ multi-potent cells. DSG2 expression is progressively decreased as a function of differentiation, with DSG2 expression absent on mature lymphocytes. DSG2 also serves as a receptor for adenoviruses and can be shed by mucosal barriers in the intestine.
Conjugate: Yellow dye
Antibody clone number: CSTEM28
Concentration: 5 µL/Test

DSG2 protein structure - 12-9159-42 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 1 Irradiation increases EnAd genome replication and particle production. ( A ) Viral genomes were quantified at 72 h post-infection (p. i.) in combined supernatant and lysate of A549, HCT116, and DLD-1 cells mock-infected or infected with EnAd-SA-GFP, Ad11p, or Ad5 at an MOI of 0.1. Cells were being irradiated at 10 Gy 24 h post-infection. Genomes were detected using hexon- (EnAd/Ad11p) or fibre-specific (Ad5) primers and probe. Data represents biological replicates +- SD. ( B ) TCID 50 /mL was quantified for supernatant harvested 5 days p. i. from A549 cells mock-infected or infected with EnAd-SA-GFP, Ad11p or Ad5 and irradiated 24 h p. i. at 0, 5, 10 or 20 Gy. Data shows mean +- SD of three experimental repeats, each point representing the mean of three biological replicates within a single experimental repeat. ( C ) CD46 or ( D ) DSG2 median fluorescence levels were measured 24 h after irradiation. A549, HCT116, or DLD-1 cells were infected with EnAd-SA-GFP at an MOI of 0.1 at 24 h before irradiation with 10 Gy. Data represents biological replicates +- SD of a single representative experiment repeated three times. ( E ) A549 cells were irradiated with 10 Gy 18 h prior to seeding in 12-well plates. At 6 h post-seeding, cells were infected with EnAd-SA-GFP at an MOI of 0 or 0.1. At 2 h post-infection, cells were washed twice before extracting and quantifying DNA for viral genomes by qPCR using hexon-specific primers and probes. Data represent biological replicates +-
Conjugate: Yellow dye

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 3 Expression level of analyzed proteins of adherens junctions (E-cadherin), desmosomes (desmoglein-2) and gap junctions (connexin-43) in SKOV-3 and SKOV-3.ip cells cultured in monolayer and 3D in vitro models. ( A , C , E ) The distributions of SKOV-3 cells (left plot) and SKOV-3.ip cells (right plot) according to fluorescence intensity detected after staining with E-cadherin-specific, desmoglein-2-specific and connexin-43-specific antibodies (red-monolayer culture, blue-spheroids, green-collagen hydrogel); ( B , D , F ) Levels of E-cadherin, desmoglein-2 and connexin-43 in monolayer and 3D models denoted as relative fluorescence values, calculated as a ratio of mean fluorescence intensity of cells stained with specific antibodies to mean fluorescence intensity of cells stained with antibodies of isotypic control. mnlr , monolayer; sph , spheroids; clgn , collagen hydrogel. ""*"" indicates significant difference in RF level from monolayer culture (ANOVA, Holm-Sidak''s multiple comparisons test, p < 0.05).
Conjugate: Yellow dye

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 1 EOC receptor expression and sensitivity to oncolytic activity to ONCOS-102 treatment. A, Flow cytometry analyses of CAR, CD46, and DSG2 receptor expression on ovarian cancer cells. At least 10 4 events were analyzed for each marker and cell line. Results represent the mean +- SEM of at least two independent experiments. Cell viability at (B) 72 hours or (C) 96 hours after ONCOS-102 treatment in five different concentrations was assessed with the MTS assay. Results are expressed as the mean percent of untreated cells +- SEM. Data represents a pool of two independent experiments run in triplicate. CAR, coxsackie and adenovirus receptor; DSG2, desmoglein-2; EOC, epithelial ovarian cancer
Conjugate: Yellow dye

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: 10.1080/2162402X.2018.1532763-F0001 Figure 1. Expression of CAR, CD46, Desmoglein-2 and PD-L1 in human melanoma cells measured by flow cytometry (at least 10 4 cells/events were analyzed by flow cytometry in one replicate experiment). Data are expressed as percentage of cells positive for the marker.
Conjugate: Yellow dye