ABIN358075

antibody from antibodies-online

Targeting: AKT1 AKT, PKB, PRKBA, RAC, RAC-alpha

Western blot (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

ABIN358075

Provider

antibodies-online

Product name

anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pSer129) antibody

Antibody type

Polyclonal

Description

Protein G Affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Reactivity

Human

Host

Rabbit

Epitope

pSer129

Vial size

0.4 mL

Storage

Store the antibody undiluted at 2-8°C for one month or (in aliquots) at-20°C for longer.

Handling

Avoid repeated freezing and thawing.

References

Submitted references

A quantitative atlas of mitotic phosphorylation.

Dephoure N, Zhou C, Villén J, Beausoleil SA, Bakalarski CE, Elledge SJ, Gygi SP
Proceedings of the National Academy of Sciences of the United States of America 2008 Aug 5;105(31):10762-7

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Myocyte shearing, myocardial sheets, and microtubules.

McCulloch AD, Omens JH
Circulation research 2006 Jan 6;98(1):1-3

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Molecular mechanisms of bitter and sweet taste transduction.

Margolskee RF
The Journal of biological chemistry 2002 Jan 4;277(1):1-4

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Akt activation by growth factors is a multiple-step process: the role of the PH domain.

Bellacosa A, Chan TO, Ahmed NN, Datta K, Malstrom S, Stokoe D, McCormick F, Feng J, Tsichlis P
Oncogene 1998 Jul 23;17(3):313-25