PA5-27869

antibody from Invitrogen Antibodies

Targeting: USP4 UNP, Unph

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-27869

Provider

Invitrogen Antibodies

Product name

USP4 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: U87-MG, MCF-7.

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of USP4 was performed by separating 30 µg of various whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a USP4 Polyclonal Antibody (Product # PA5-27869) at a dilution of 1:500 and a HRP-conjugated anti-rabbit IgG secondary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using USP4 Polyclonal Antibody (Product # PA5-27869). Sample (30 µg of whole cell lysate). Lane A: U87-MG . 7.5% SDS PAGE. USP4 Polyclonal Antibody (Product # PA5-27869) diluted at 1:1,000.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using USP4 (Product # PA5-27869) antibody at 1:500 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

USP4 Polyclonal Antibody detects USP4 protein at cytoplasm in mouse muscle by immunohistochemical analysis. Sample: Paraffin-embedded mouse muscle. USP4 Polyclonal Antibody (Product # PA5-27869) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.