TA809083

antibody from OriGene

Targeting: AFP FETA, HPAFP

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA809083

Provider

OriGene

Proper citation

OriGene Cat#TA809083, RRID:AB_2629003

Product name

AFP mouse monoclonal antibody, clone OTI4D8 (formerly 4D8)

Antibody type

Monoclonal

Description

AFP mouse monoclonal antibody, clone OTI4D8 (formerly 4D8)

Host

Mouse

Conjugate

Unconjugated

Epitope

AFP

Isotype

IgG

Antibody clone number

OTI4D8

Vial size

100 µl

Concentration

1.00mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AFP (RC206622, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AFP.(1:2000)

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunofluorescent staining of HT29 cells using anti-AFP mouse monoclonal antibody (TA809083).(1:100)

Validation comment

IF

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-AFP mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120?‚°C for 3 min, TA809083)(1:150)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-AFP mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA809083)(1:150)

Validation comment

IHC