Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- AF5369 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse alpha-Fetoprotein/AFP Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects mouse alpha-Fetoprotein/AFP in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with human alpha-Fetoprotein is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P02772
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Mice lacking RAP1 show early onset and higher rates of DEN-induced hepatocellular carcinomas in female mice.
Intrathymic Notch3 and CXCR4 combinatorial interplay facilitates T-cell leukemia propagation.
Kupffer Cell-Derived Tnf Triggers Cholangiocellular Tumorigenesis through JNK due to Chronic Mitochondrial Dysfunction and ROS.
Hepatic loss of miR-122 predisposes mice to hepatobiliary cyst and hepatocellular carcinoma upon diethylnitrosamine exposure.
Ferrara-Romeo I, Martínez P, Blasco MA
PloS one 2018;13(10):e0204909
PloS one 2018;13(10):e0204909
Intrathymic Notch3 and CXCR4 combinatorial interplay facilitates T-cell leukemia propagation.
Ferrandino F, Bernardini G, Tsaouli G, Grazioli P, Campese AF, Noce C, Ciuffetta A, Vacca A, Besharat ZM, Bellavia D, Screpanti I, Felli MP
Oncogene 2018 Dec;37(49):6285-6298
Oncogene 2018 Dec;37(49):6285-6298
Kupffer Cell-Derived Tnf Triggers Cholangiocellular Tumorigenesis through JNK due to Chronic Mitochondrial Dysfunction and ROS.
Yuan D, Huang S, Berger E, Liu L, Gross N, Heinzmann F, Ringelhan M, Connor TO, Stadler M, Meister M, Weber J, Öllinger R, Simonavicius N, Reisinger F, Hartmann D, Meyer R, Reich M, Seehawer M, Leone V, Höchst B, Wohlleber D, Jörs S, Prinz M, Spalding D, Protzer U, Luedde T, Terracciano L, Matter M, Longerich T, Knolle P, Ried T, Keitel V, Geisler F, Unger K, Cinnamon E, Pikarsky E, Hüser N, Davis RJ, Tschaharganeh DF, Rad R, Weber A, Zender L, Haller D, Heikenwalder M
Cancer cell 2017 Jun 12;31(6):771-789.e6
Cancer cell 2017 Jun 12;31(6):771-789.e6
Hepatic loss of miR-122 predisposes mice to hepatobiliary cyst and hepatocellular carcinoma upon diethylnitrosamine exposure.
Hsu SH, Wang B, Kutay H, Bid H, Shreve J, Zhang X, Costinean S, Bratasz A, Houghton P, Ghoshal K
The American journal of pathology 2013 Dec;183(6):1719-1730
The American journal of pathology 2013 Dec;183(6):1719-1730
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Mouse alpha-Fetoprotein/AFP by Western Blot. Western blot shows lysates of mouse plasma and rat placenta tissue. PVDF membrane was probed with 1 µg/mL of Mouse alpha-Fetoprotein/AFP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5369) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for alpha-Fetoprotein/AFP at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.