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- Antigen structure
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- Validations
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- Product number
- IC1368P - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse alpha-Fetoprotein/AFP PE-conjugated Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human alpha-Fetoprotein/AFP in direct ELISAs and Western blots.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 189502
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
Submitted references Generation of iPSCs from Jaw Periosteal Cells Using Self-Replicating RNA.
Umrath F, Steinle H, Weber M, Wendel HP, Reinert S, Alexander D, Avci-Adali M
International journal of molecular sciences 2019 Apr 3;20(7)
International journal of molecular sciences 2019 Apr 3;20(7)
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of alpha-Fetoprotein/AFP in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human/Mouse alpha-Fetoprotein/AFP PE-conjugated Monoclonal Antibody (Catalog # IC1368P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- alpha-Fetoprotein/AFP Specificity is Shown by Flow Cytometry in Knockout Cell Line. alpha-Fetoprotein/AFP knockout HepG2 hepatocellular carcinoma cell line was stained with PE-conjugated Mouse Anti-Human alpha-Fetoprotein/AFP Monoclonal Antibody (Catalog # IC1368P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). No staining in the alpha-Fetoprotein/AFP knockout HepG2 cell line was observed. View our protocol for Staining Membrane-associated Proteins.
