Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [5]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-34946 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TNFRSF11B Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: human plasma, TNFRSF11B-transfected 293T.
- Concentration
- 1.85 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot analysis of TNFRSF11B using 30 µg Raji whole cell lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a TNFRSF11B polyclonal antibody (Product # PA5-34946) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of TNFRSF11B using 50 µg mouse heart lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a TNFRSF11B polyclonal antibody (Product # PA5-34946) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of TNFRSF11B was performed by separating 30 µg of non-transfected (–) and transfected (+) 293T whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a TNFRSF11B Polyclonal Antibody (Product # PA5-34946) at a dilution of 1:1500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using TNFRSF11B Polyclonal Antibody (Product # PA5-34946). Human plasma (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with TNFRSF11B Polyclonal Antibody (Product # PA5-34946) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of TNFRSF11B was performed by separating 30 µg of Human plasma by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a TNFRSF11B Polyclonal Antibody (Product # PA5-34946) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- TNFRSF11B Polyclonal Antibody detects TNFRSF11B protein at cytosol on Cal27 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded Cal27 xenograft. TNFRSF11B Polyclonal Antibody (Product # PA5-34946) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.