AF459

antibody from R&D Systems

Targeting: TNFRSF11B OCIF, OPG, TR1

Immunohistochemistry (Supportive data in Antibodypedia)

Blocking/Neutralizing (Supportive data in Antibodypedia)

Antibody Data

Product number

AF459

Provider

R&D Systems

Product name

Mouse Osteoprotegerin/TNFRSF11B Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects mouse Osteoprotegerin in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant human Osteoprotegerin is observed.

Reactivity

Mouse

Host

Goat

Conjugate

Unconjugated

Antigen sequence

Q6PI12


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Morc3 mutant mice exhibit reduced cortical area and thickness, accompanied by altered haematopoietic stem cells niche and bone cell differentiation.

Jadhav G, Teguh D, Kenny J, Tickner J, Xu J
Scientific reports 2016 May 18;6:25964

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TRAIL stimulates proliferation of vascular smooth muscle cells via activation of NF-kappaB and induction of insulin-like growth factor-1 receptor.

Kavurma MM, Schoppet M, Bobryshev YV, Khachigian LM, Bennett MR
The Journal of biological chemistry 2008 Mar 21;283(12):7754-62

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JunB as a downstream mediator of PTHrP actions in cementoblasts.

Berry JE, Ealba EL, Pettway GJ, Datta NS, Swanson EC, Somerman MJ, McCauley LK
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 2006 Feb;21(2):246-57

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Bacteria induce osteoclastogenesis via an osteoblast-independent pathway.

Jiang Y, Mehta CK, Hsu TY, Alsulaimani FF
Infection and immunity 2002 Jun;70(6):3143-8

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Bacteria induce osteoclastogenesis via an osteoblast-independent pathway.

Jiang Y, Mehta CK, Hsu TY, Alsulaimani FF
Infection and immunity 2002 Jun;70(6):3143-8

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Estrogen inhibition of PTH-stimulated osteoclast formation and attachment in vitro: involvement of both PKA and PKC.

Liu BY, Wu PW, Bringhurst FR, Wang JT
Endocrinology 2002 Feb;143(2):627-35

Immunohistochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Osteoprotegerin/TNFRSF11B in Mouse Stomach. Osteoprotegerin/TNFRSF11B was detected in perfusion fixed frozen sections of mouse stomach using Goat Anti-Mouse Osteoprotegerin/TNFRSF11B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF459) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to glandular epithelial cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Blocking/Neutralizing

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Osteoprotegerin/TNFRSF11B Inhibition of TRAIL/TNFSF10-induced Cytotoxicity and Neutralization by Mouse Osteoprotegerin/TNFRSF11B Antibody. In the presence of the metabolic inhibitor actinomycin D (1 µg/mL), Recombinant Mouse OPG/TNFRSF11B Fc Chimera (Catalog # 459-MO) inhibits Recombinant Human TRAIL/TNFSF10 (Catalog # 375-TL) induced cytotoxicity in the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Under these conditions, inhibition of Recombinant Human TRAIL/TNFSF10 (20 ng/mL) activity elicited by Recombinant Mouse OPG/TNFRSF11B Fc Chimera (0.1 µg/mL) is neutralized (green line) by increasing concentrations of Mouse OPG/TNFRSF11B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF459). The ND₅₀ is typically 0.15-0.9 µg/mL.