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Immunoprecipitation
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [1]
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- Product number
- GTX18051 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX18051, RRID:AB_423045
- Product name
- OPG antibody [bony-1]
- Antibody type
- Monoclonal
- Reactivity
- Human
- Host
- Rat
- Storage
- Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles.
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Supportive validation
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- GeneTex (provider)
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- Experimental details
- FACS analysis of cells with MAb to OPG (human) (GTX18051). HEK 293T cells (5x105) were mock transfected (thin line) or transfected with an expression plasmid enabling surface expression of the TNFR homology domain of human OPG (thick line). Cells were incubated on ice for 30 min. in 50?l FACS buffer (PBS, 5% fetal calf serum, 0.02% azide) containing 1?g/ml of MAb to OPG (human) (Bony-1) (GTX18051). After washing in FACS buffer, RPE-conjugated antibody to rat IgG was added. Cells were incubated on ice for 30 min, washed and then analyzed by flow cytometry.
