Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [5]
- Immunohistochemistry [1]
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Validation data
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- Product number
- GTX127948 - Provider product page
- Provider
- GeneTex
- Product name
- OPG antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
Submitted references Butyrate Stimulates Histone H3 Acetylation, 8-Isoprostane Production, RANKL Expression, and Regulated Osteoprotegerin Expression/Secretion in MG-63 Osteoblastic Cells.
Chang MC, Chen YJ, Lian YC, Chang BE, Huang CC, Huang WL, Pan YH, Jeng JH
International journal of molecular sciences 2018 Dec 17;19(12)
International journal of molecular sciences 2018 Dec 17;19(12)
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- OPG antibody detects TNFRSF11B protein by Western blot analysis.A. 30 µg Raji whole cell lysate/extract10 % SDS-PAGEOPG antibody (GTX127948) dilution: 1:1000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- OPG antibody detects TNFRSF11B protein by Western blot analysis.A. 50 µg mouse heart lysate/extract10 % SDS-PAGEOPG antibody (GTX127948) dilution: 1:1000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Mouse tissue extract (50 ?g) was separated by 10% SDS-PAGE, and the membrane was blotted with OPG antibody (GTX127948) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Human plasma (30 ?g) was separated by 7.5% SDS-PAGE, and the membrane was blotted with OPG antibody (GTX127948) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Mouse tissue extract (50 ?g) was separated by 10% SDS-PAGE, and the membrane was blotted with OPG antibody (GTX127948) diluted at 1:1000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- OPG antibody detects TNFRSF11B protein at cytosol on Cal27 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded Cal27 xenograft. OPG antibody (GTX127948) dilution: 1:500.